IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for <i>Plasmodium vivax</i> Infections.

ABSTRACT:

Introduction

The tools to distinguish relapse from reinfection are needed in malaria-endemic areas. We evaluated seroprevalence against sets of specific peptides to the block 2 region of Plasmodium vivax-merozoite surface protein-1 (PvMSP1) to detect parasite clones.

Methods

We applied amplicon deep sequencing (ADS) of block 2 region of the MSP-1 gene (pvmsp1) to determine cocirculating parasite clones within eight P. vivax-infected individuals. Based on this, a seroprevalence of IgM and IgG antibodies against sets of peptides of different block-2 haplotypes was validated. After, we evaluated the seroprevalence in plasma of 72 pregnant women, from which 31 had recurrent P. vivax infections.

Results

ADS revealed one block 2 haplotype clone i

SUBMITTER: Batalha FA 

PROVIDER: S-EPMC11775398 | biostudies-literature | 2024 Oct-Dec

REPOSITORIES: biostudies-literature