NpCIPK6-NpSnRK1 module facilitates intersubgeneric hybridization barriers in water lily (<i>Nymphaea</i>) by reducing abscisic acid content.

ABSTRACT: Prefertilization hybridization barriers are the main causes of intersubgeneric hybridization challenges in water lily. However, the mechanism underlying low compatibility between pollen and stigma of water lily remains unclear. This study demonstrates that CBL-interacting protein kinase 6 (CIPK6) responded to the signaling exchange between incompatible pollen and stigma through interactions with SNF1-related kinase 1 (SnRK1) and promotes the accumulation of SnRK1 protein. Activated SnRK1 interacted with 9-cis-epoxycarotenoid dioxygenase 2 (NCED2) to promote its degradation, thereby inhibiting abscisic acid (ABA) synthesis. A decrease in ABA content in the stigma impaired the ABA-mediated removal of reactive oxygen species (ROS), ultimately resulting in the rejection of the incompati

SUBMITTER: Zhou P 

PROVIDER: S-EPMC11775591 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature