Single-Virus Microscopy of Biochemical Events in Viral Entry.

ABSTRACT: Cell entry by enveloped viruses involves a set of multistep, multivalent interactions between viral and host proteins as well as manipulation of nanoscale membrane mechanics by these interacting partners. A mechanistic understanding of these events has been challenging due to the complex nature of the interactions and the event-to-event heterogeneity involved. Single-virus microscopy has emerged as a powerful technique to probe viral binding and fusion kinetics. Single-event distributions compiled from individual viral particle measurements have enabled estimates of protein stoichiometry at fusion interfaces, a better understanding of the rate-limiting steps for fusion, and a more robust identification of the biochemical regulatory factors for viral entry. Recent technical advances have ma

SUBMITTER: Cervantes M 

PROVIDER: S-EPMC11775682 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature