Simultaneous Live Mapping of pH and Hydrogen Peroxide Fluctuations in Autophagic Vesicles.

ABSTRACT: Hydrogen peroxide (H₂O₂) plays a critical role in the regulation and progress of autophagy, an essential recycling process that influences cellular homeostasis and stress response. Autophagy is characterized by the formation of intracellular vesicles analogous to recycle "bags" called autophagosomes, which fuse with lysosomes to form autolysosomes, eventually ending up as lysosomes. We have developed two novel autophagic vesicle-targeted peptide-based sensors, ROSA for H₂O₂ and pHA for pH, to simultaneously track H₂O₂ and pH dynamics within autophagic vesicles as autophagy advances. Since pH values progressively decrease within autophagic vesicles with the progress of autophagy, we utilized information on vesic

SUBMITTER: Kahali S 

PROVIDER: S-EPMC11775711 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature