Ionic silver coating of orthopedic implants may impair osteogenic differentiation and mineralization.

ABSTRACT: Silver (Ag) possesses potent antimicrobial properties and is used as a coating for medical devices. The impact of silver ions released from orthopedic implants on the differentiation and osteoid formation of different osteogenic cells has yet to be systematically studied. In the present study, human mesenchymal stem cells (hMSCs) and primary human osteoblasts (hOBs) were exposed to different static Ag⁺ concentrations (0, 0.5, 1.0 or 1.5 ppm) or dynamic Ag⁺ concentrations (range 0 to 0.7 ppm) that simulated the temporal release pattern from a Ag-nitrate coating of trabecular titanium (TLSN). Cell morphology was investigated by phase contrast and fluorescence microscopy. The activities of alkaline phosphatase (ALP) and lactate dehydrogenase, osteogenic gene expression (

SUBMITTER: Kontakis MG 

PROVIDER: S-EPMC11775765 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature