The Hansen's baccata #2 gene Rvi12_Cd5 confers scab resistance to the susceptible apple cultivar "Gala Galaxy".

ABSTRACT: To enhance the breeding of new scab-resistant apple cultivars, a comprehensive understanding of the mechanisms governing major scab resistance genes is essential. Rvi12_Cd5 was previously identified as the best candidate gene for the Rvi12 scab resistance of the crab apple "Hansen's baccata #2" by gene prediction and in silico analysis. In the present study, Rvi12_Cd5 was used to transform the scab-susceptible apple cultivar "Gala Galaxy." Two constructs were prepared: the first carrying Rvi12_Cd5 under the control of a 35S promoter and E9 terminator, and the second carrying Rvi12_Cd5 under the control of its native promoter and terminator. All the transgenic lines were analyzed for T-DNA integration, copy number, and expression of Rvi12_Cd5 and phenotypically evaluated for scab resistance

SUBMITTER: Yousaf A 

PROVIDER: S-EPMC11776037 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature