The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice.

ABSTRACT:

Background

The high-frequency induction rate of haploid is crucial for double haploid (DH) breeding. The combination of multiple haploid-induced genes, such as ZmPLA1/MATL/NLD and ZmDMP, can synergistically enhance the haploid induction rate (HIR) in maize. However, the potential synergistic effects between OsMATL and OsDMP genes in rice remain unclear.

Results

Knocking out OsMATL in both HuaHang No.48 (HH48) and Nipponbare (NIP) cultivars resulted in reduced seed setting rate (SSR) and haploid induction (HI). Notably, in this study, the HI capacity of OsMATL knockout mutants in indica rice surpassed that of japonica rice knockout mutants, with the proton active site in the third exon exhibiting a higher HIR compared to the first and fourth exons. Furthermore, when OsDMP1 or

SUBMITTER: Liang S 

PROVIDER: S-EPMC11776126 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature