Neuropilin1-dependent paracrine signaling of cancer cells mediated by miRNA exosomal cargo.

ABSTRACT:

Background

Neuropilin-1 (NRP1) is a transmembrane protein involved in surface receptor complexes for a variety of extracellular signals. NRP1 expression in human cancers is associated with prominent angiogenesis and advanced progression stage. However, the molecular mechanisms underlying NRP1 activity in the tumor microenvironment remain unclear. Notably, diffusible forms of NRP1 in the extracellular space have been reported, but their functional role is poorly understood.

Methods

Extracellular vesicles (EV) were isolated from conditioned media of diverse cancer cells. The quality of exosome-enriched preparations was validated by the presence of specific markers in western blotting, as well as by light scattering and nanoparticle tracking analysis. Wound healing, transwell,

SUBMITTER: Palazzo C 

PROVIDER: S-EPMC11776261 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature