Computational identification and experimental validation of novel <i>Saccharum officinarum</i> microRNAs along with their targets through RT-PCR approach.

ABSTRACT: Various metabolic and cell signaling processes impact the functions of sugarcane plant cells. MicroRNAs (miRNAs) play critical regulatory roles in enhancing yield and providing protection against various stressors. This study seeks to identify and partially characterize several novel miRNAs in sugarcane using in silico tools, while also offering a preliminary assessment of their functions. This was accomplished by predicting novel conserved miRNAs in sugarcane plants using a variety of genomics-based techniques like BLASTn, MFOLD, psRNA Target, sequence logo, Weblogo, primer-3, etc. and annotated using miRBase and NCBI. For validation, RT-PCR method was used along with agarose gel. After the preparation of fourteen randomly chosen primers, they were validated by RT-PCR. Accordingly,

SUBMITTER: Baqi A 

PROVIDER: S-EPMC11776470 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature