Lipid droplet-associated proteins in alcohol-associated fatty liver disease: A proteomic approach.

ABSTRACT:

Background

The earliest manifestation of alcohol-associated liver disease (ALD) is steatosis characterized by deposition of fat in specialized organelles called lipid droplets (LDs). While alcohol administration causes a rise in LD numbers in the hepatocytes, little is known regarding their characteristics that allow their accumulation and size to increase. The aim of the present study is to gain insights into underlying pathophysiological mechanisms by investigating the ethanol-induced changes in hepatic LD proteome as a function of LD size.

Methods

Adult male Wistar rats (180-200 g BW) were fed with ethanol liquid diet for 6 weeks. At sacrifice, large-, medium-, and small-sized hepatic LD subpopulations (LD1, LD2, and LD3, respectively) were isolated and subjected to morph

SUBMITTER: Perumal SK 

PROVIDER: S-EPMC11778054 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature