Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2).

ABSTRACT:

Background

The number of patients candidate to yeast infection has increased during the last years. Also the variety of species of clinical importance has increased. Correct species identification is often important for efficient therapy, but is based on phenotypic features and is sometimes time-consuming and depends largely on the expertise of technicians. Therefore, we evaluated the feasability of PCR-based amplification of the Internally Transcribed Spacer region 2, followed by fragment size analysis on the ABI Prism 310 for the identification of clinically important yeasts.

Results

A rapid DNA-extraction method, based on simple boiling freezing was introduced. Of the 25 species tested, 22 could be identified unambiguously by scoring the length of the ITS2-region. No dist

SUBMITTER: De Baere T 

PROVIDER: S-EPMC117793 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature