Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species.

ABSTRACT:

Background

Mycoplasmas are present worldwide in a large number of animal hosts. Due to their small genome and parasitic lifestyle, Mycoplasma spp. require complex isolation media. Nevertheless, already over 100 different species have been identified and characterized and their number increases as more hosts are sampled. We studied the applicability of amplified rDNA restriction analysis (ARDRA) for the identification of all 116 acknowledged Mycoplasma species and subspecies.

Methods

Based upon available 16S rDNA sequences, we calculated and compared theoretical ARDRA profiles. To check the validity of these theoretically calculated profiles, we performed ARDRA on 60 strains of 27 different species and subspecies of the genus Mycoplasma.

Results

In silico digestion wit

SUBMITTER: Stakenborg T 

PROVIDER: S-EPMC1177949 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature