Single nucleotide polymorphism-based visual identification of <i>Rhodiola crenulata</i> using the loop-mediated isothermal amplification technique.

ABSTRACT:

Introduction

Rhodiola crenulata (Hook.f. & Thomson) H.Ohba, a member of the Crassulaceae family, is a traditional Chinese medicine recognized as the original source of Rhodiolae Crenulatae Radix et Rhizoma in the 2020 edition of the China Pharmacopoeia. It has been widely used in both Asia and Europe to enhance stress resistance and reduce fatigue. However, the classification of Rhodiola species can lead to confusion, raising safety concerns in the herbal medicine market.

Methods

The cleaved amplified polymorphic sequence (CAPS) RT-PCR was used to identify the single nucleotide polymorphism (SNP) sites, based on which the loop-mediated isothermal amplification (LAMP) was employed to develop the method in Rh. crenulata identification. The specific loop ba

SUBMITTER: Hao L 

PROVIDER: S-EPMC11779703 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature