Clinical evaluation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections: a prospective study.

ABSTRACT:

Background

Though droplet digital PCR (ddPCR) has emerged as a promising tool for early pathogen detection in bloodstream infections (BSIs), more studies are needed to support its clinical application widely due to different ddPCR platforms with discrepant diagnostic performance. Additionally, there is still a lack of clinical data to reveal the association between pathogen loads detected by ddPCR and corresponding BSIs.

Methods

In this prospective study, 173 patients with suspected BSIs were enrolled. A multiplex ddPCR assay was used to detect 18 pathogens. The results of ddPCR testing were evaluated in comparison with blood cultures (BCs) and clinical diagnosis. Taking BC as the gold standard, receiver operating characteristic curve and Cohen's kappa agreement were used to

SUBMITTER: Peng Y 

PROVIDER: S-EPMC11779721 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature