Preparation and evaluation of <i>Brucella</i> T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology.

ABSTRACT:

Introduction

Brucellosis, a significant zoonotic infectious disease, poses a global health threat. Accurate and efficient diagnosis is crucial for prevention, control, and treatment of brucellosis. VirB proteins, components of the Type IV secretion system (T4SS) in Brucella, play a pivotal role in bacterial virulence and pathogenesis but have been understudied for their diagnostic potential.

Methods

Tandem Mass Tag (TMT) proteomics technology was utilized to identify highly expressed VirB proteins from wild-type Brucella strains. Recombinant T4SS proteins were prepared, and an indirect ELISA method was established for serological diagnosis of human brucellosis.

Results

Seven T4SS proteins (rVirB3, rVirB4, rVirB9, rBMEII0036, rVirB8, rVirB11, and rVirB10)

SUBMITTER: Wu Q 

PROVIDER: S-EPMC11779724 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature