Combination of Two-Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in <i>Lipomyces starkeyi</i>.

ABSTRACT: The oleaginous yeast Lipomyces starkeyi is recognized for its remarkable lipid accumulation under nitrogen-limited conditions. However, precise control of microbial lipid production in L. starkeyi remains challenging due to the complexity of nutrient media. We developed a two-stage fed-batch fermentation process using a well-defined synthetic medium in a 5-L bioreactor. In the first stage, the specific growth rate was maintained at a designated level by maximizing the cell density through optimizing the feeding rate, molar carbon-to-nitrogen (C/N) ratio, and phosphate concentration in feeding media, achieving a high cell density of 213 ± 10 × 10⁷ cells mL^-1. In the second stage, we optimized the molar C/N ratio in the feeding medium for lipid production

SUBMITTER: Wu CC 

PROVIDER: S-EPMC11779743 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature