Chemical- and photo-activation of protein-protein thiol-ene coupling for protein profiling.

ABSTRACT: The thiol-ene reaction between an alkene and a thiol can be exploited for selective labelling of cysteine residues in protein profiling applications. Here, we explore thiol-ene activation in systems from chemical models to complex cellular milieus, using UV, visible wavelength and redox initiators. Initial studies in chemical models required an oxygen-free environment for efficient coupling and showed very poor activation when using a redox initiator. When thiol-ene activation was performed in protein and cell lysate models, all three initiation methods were successful. Faster thiol-ene reaction was observed as the cysteine and alkene were brought into proximity by a binding event prior to activation, leading to quicker adduct formation in the protein model system than the chemical models.

SUBMITTER: Campaniҫo A 

PROVIDER: S-EPMC11779957 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature