Amplification-Free Attomolar Detection of Short Nucleic Acids with Upconversion Luminescence: Eliminating Nonspecific Binding by Hybridization Complex Transfer.

ABSTRACT: The anti-Stokes emission of photon upconversion nanoparticles (UCNPs) facilitates their use as labels for ultrasensitive detection in biological samples as infrared excitation does not induce autofluorescence at visible wavelengths. The detection of extremely low-abundance analytes, however, remains challenging as it is impossible to completely avoid nonspecific binding of label conjugates. To overcome this limitation, we developed a novel hybridization complex transfer technique using UCNP labels to detect short nucleic acids directly without target amplification. The assay involves capturing the target-label complexes on an initial solid phase, then using releasing oligonucleotides to specifically elute only the target-UCNP complexes and recapturing them on another solid phase. The nonsp

SUBMITTER: Macala J 

PROVIDER: S-EPMC11780573 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature