Lab-on-the-Needles: A Microneedle Patch-Based Mobile Unit for Highly Sensitive <i>Ex Vivo</i> and <i>In Vivo</i> Detection of Protein Biomarkers.

ABSTRACT: Detection of biomarkers associated with physiological conditions provides critical insights into healthcare and disease management. However, challenges in sampling and analysis complicate the detection and quantification of protein biomarkers within the epidermal layer of the skin and in viscous liquid biopsy samples. Here, we present the "Lab-on-the-Needles" concept, utilizing a microneedle patch-based sensing box (MNP-based SenBox) for mobile healthcare applications. This system facilitates the rapid capture of protein biomarkers directly from the in situ epidermal layer of skin or liquid biopsies, followed by on-needle analysis for immediate assessment. The integration of horseradish peroxidase-incorporated zeolitic imidazolate framework-8 (HRP@ZIF-8) as a sensitive and stable si

SUBMITTER: Hsu YP 

PROVIDER: S-EPMC11781025 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature