Dual DNA replication modes: varying fork speeds and initiation rates within the spatial replication program in Xenopus.

ABSTRACT: Large vertebrate genomes duplicate by activating tens of thousands of DNA replication origins, irregularly spaced along the genome. The spatial and temporal regulation of the replication process is not yet fully understood. To investigate the DNA replication dynamics, we developed a methodology called RepliCorr, which uses the spatial correlation between replication patterns observed on stretched single-molecule DNA obtained by either DNA combing or high-throughput optical mapping. The analysis revealed two independent spatiotemporal processes that regulate the replication dynamics in the Xenopus model system. These mechanisms are referred to as a fast and a slow replication mode, differing by their opposite replication fork speed and rate of origin firing. We found that Polo-like kinase 1

SUBMITTER: Ciardo D 

PROVIDER: S-EPMC11781033 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature