A non-conditioned bone marrow transplantation mouse model to study clonal hematopoiesis and myeloid malignancies.

ABSTRACT: Clonal hematopoiesis of indeterminate potential (CHIP) is a condition where blood or bone marrow cells carry mutations associated with hematological malignancies. Individuals with CHIP have an increased risk of developing hematological malignancies, atherosclerotic cardiovascular disease, and all-cause mortality. Bone marrow transplantation (BMT) of cells carrying CHIP mutations into irradiated mice are useful procedures to investigate the dynamics of clonal expansion and potential therapeutic strategies, but myeloablative conditioning can induce confounding effects. We established a non-conditioned BMT model using C57BL/6J-Kit^W-41J/J (W⁴¹) recipient mice to overcome the unwanted effects of irradiation. Conditional Tet2 deletion using tamoxifen was used to obtain Tet2

SUBMITTER: Bentivegna S 

PROVIDER: S-EPMC11781034 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature