CCDC154 knockdown inhibits growth of liver cancer via suppressing expression of Snail.

ABSTRACT:

Objective

The effect of coiled-coil domain-containing 154 (CCDC154) in liver cancer (LC) remains unexplored. The objective of this study was to investigate the role of CCDC154 in LC and its underlying mechanism.

Methods

The analysis of CCDC154 expression and prognosis was performed using UALCAN, Human Protein Atlas and Kaplan-Meier plotter websites. Protein expression was measured using Western blotting assay. Lentivirus was used to silence CCDC154 expression in LC cells. The proliferation and apoptosis of LC cells was evaluated by cell counting assay, colony formation assay and flow cytometry. The migration and invasion of LC cells were investigated using scratch wound-healing assay and Transwell assay.

Results

The results showed that CCDC154 was highly expressed in

SUBMITTER: Ma R 

PROVIDER: S-EPMC11781066 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature