Separation of 100 nm-sized nanoparticles using a poly-Lys-modified monolith column.

ABSTRACT: Nanoparticles (approximately 100 nm in diameter) composed of lipid layers containing drugs or biologically active substances are attracting increasing attention in various fields, including medicine, as well as for signal transduction between cells. However, the separation of such nanoparticles via conventional HPLC is challenging, often resulting in the clogging and collapse of nanoparticles, as well as a low separation efficiency. Thus far, no HPLC column capable of efficiently separating two types of 100 nm-sized nanoparticles in a short time has been reported. In this study, a poly-Lys-modified monolithic column was prepared for nanoparticle analysis via HPLC using anticancer drug-encapsulated nanoparticles (Doxil®) and small extracellular vesicles (sEVs) to examine their

SUBMITTER: Kato M 

PROVIDER: S-EPMC11781079 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature