Biophysical modeling and experimental analysis of the dynamics of C. elegans body-wall muscle cells.

ABSTRACT: This study combines experimental techniques and mathematical modeling to investigate the dynamics of C. elegans body-wall muscle cells. Specifically, by conducting voltage clamp and mutant experiments, we identify key ion channels, particularly the L-type voltage-gated calcium channel (EGL-19) and potassium channels (SHK-1, SLO-2), which are crucial for generating action potentials. We develop Hodgkin-Huxley-based models for these channels and integrate them to capture the cells' electrical activity. To ensure the model accurately reflects cellular responses under depolarizing currents, we develop a parallel simulation-based inference method for determining the model's free parameters. This method performs rapid parallel sampling across high-dimensional parameter spaces, fitting the model

SUBMITTER: Du X 

PROVIDER: S-EPMC11781704 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature