Structural Mapping of Protein Aggregates in Live Cells Modeling Huntington's Disease.

ABSTRACT: While protein aggregation is a hallmark of many neurodegenerative diseases, acquiring structural information on protein aggregates inside live cells remains challenging. Traditional microscopy does not provide structural information on protein systems. Routinely used fluorescent protein tags, such as Green Fluorescent Protein (GFP), might perturb native structures. Here, we report a counter-propagating mid-infrared photothermal imaging approach enabling mapping of secondary structure of protein aggregates in live cells modeling Huntington's disease. By comparing mid-infrared photothermal spectra of label-free and GFP-tagged huntingtin inclusions, we demonstrate that GFP fusions indeed perturb the secondary structure of aggregates. By implementing spectra with small spatial step for dissect

SUBMITTER: Guo Z 

PROVIDER: S-EPMC11781839 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature