Optogenetic control of gene expression in the cyanobacterium Synechococcus sp. PCC 7002.

ABSTRACT: Photosynthetic cyanobacteria can be utilised in biotechnology as environmentally sustainable cell factories to convert CO₂ into a diverse range of biochemicals. However, a lack of molecular tools available for precise and dynamic control of gene expression hinders metabolic engineering and contributes to low product titres. Optogenetic tools enable light-regulated control of gene expression with high tunability and reversibility. To date, their application in cyanobacteria is limited and transferability between species remains unclear. In this study, we expressed the blue light-repressible YF1/FixJ and the green/red light-responsive CcaS/CcaR systems in Synechococcus sp. PCC 7002 and characterised their performance using GFP fluorescence assays and qRT-PCR. The YF1/FixJ s

SUBMITTER: Forbes L 

PROVIDER: S-EPMC11782128 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature