Photosynthesis in Synechocystis sp. PCC 6803 is not optimally regulated under very high CO₂.

ABSTRACT: One strategy for CO₂ mitigation is using photosynthetic microorganisms to sequester CO₂ under high concentrations, such as in flue gases. While elevated CO₂ levels generally promote growth, excessively high levels inhibit growth through uncertain mechanisms. This study investigated the physiology of the cyanobacterium Synechocystis sp. PCC 6803 under very high CO₂ concentrations and yet stable pH around 7.5. The growth rate of the wild type (WT) at 200 µmol photons m^-2 s^-1 and a gas phase containing 30% CO₂ was 2.7-fold lower compared to 4% CO₂. Using a CRISPR interference mutant library, we identified genes that, when repressed, either enhanced or impaired growth under 30% or 4% CO₂. Repress

SUBMITTER: Carrasquer-Alvarez E 

PROVIDER: S-EPMC11782454 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature