Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain.

ABSTRACT: Azurin, a bacterial blue-copper protein, has garnered significant attention as a potential anticancer drug in recent years. Among twenty Pseudomonas aeruginosa isolates, we identified one isolate that demonstrated potent and remarkable azurin synthesis using the VITEK 2 system and 16S rRNA sequencing. The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active. Furthermore, crude azurin protein was extracted, precipitated using 70% ammonium sulfate, dialyzed, and subjected to purification using carboxymethyl-Sephadex in affinity chromatography as a cheap method for purification. The partially purified azurin protein was characteri

SUBMITTER: Zaghloul NA 

PROVIDER: S-EPMC11782508 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature