Advancing ex vivo functional whole-organ prostate gland model for regeneration and drug screening.

ABSTRACT: Model organisms are vital for biomedical research and drug testing but face high costs, complexity, and ethical issues. While newer techniques like organoids and assembloids have shown improvements, they still remain inadequate in addressing all research needs. In this study, we present a new method for maintaining the prostate gland of the earthworm, Eudrilus eugeniae ex vivo and examine its potential for regeneration and drug screening. We successfully maintained the earthworm prostate gland in cell culture media for over 200 days, with observed beating behavior confirming its viability. Apoptotic staining and histological analysis show no significant changes, indicating that the prostate gland remains stable. However, significant overexpression of H3 and H2AX on the 10th and 50th days s

SUBMITTER: Subbiahanadar Chelladurai K 

PROVIDER: S-EPMC11782681 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature