Single-cell analysis reveals islet autoantigen's immune activation in type 1 diabetes patients.

ABSTRACT: In this study, we used single-cell sequencing, which can comprehensively detect the type and number of transcripts per cell, to efficiently stimulate peripheral blood mononuclear cells of type 1 diabetic patients with overlapping peptides of GAD, IA-2, and insulin antigens, and performed gene expression analysis by single-cell variable-diversity-joining sequencing and T-cell receptor repertoire analysis. Twenty male patients with type 1 diabetes mellitus participating in the KAMOGAWA-DM cohort were included. Four of them were randomly selected for BD Rhapsody system after reacting peripheral blood mononuclear cells with overlapping peptides of GAD, IA-2, and insulin antigen. Peripheral blood mononuclear cells were clustered into CD8⁺ T cells, CD4⁺ T cells, granulocyte

SUBMITTER: Okamura T 

PROVIDER: S-EPMC11782777 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature