Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3.

ABSTRACT: GABA-producing probiotics present promising opportunities for developing functional foods. Carbon sources have been identified as a critical influence on GABA production. Therefore, this study investigated the holistic metabolic responses and GABA biosynthesis to various carbon sources of Lactiplantibacillus pentosus 9D3, a proficient GABA producer, using a genome-wide transcriptomic approach. The analysis revealed 414 genes with differential expression responses to altering carbon sources, i.e., glucose, sucrose, and lactose, notably sugar phosphotransferase systems (PTS) (11 genes), indicating carbon source-mediated transcriptional change patterns in L. pentosus 9D3. The integration of transcriptome data with a genome-scale metabolic network (GSMN) revealed that L. pento

SUBMITTER: Raethong N 

PROVIDER: S-EPMC11782964 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature