Biodesulfurization enhancement via targeted re-insertion of the flavin reductase dszD in the genome of the model strain Rhodococcus qingshengii IGTS8.

ABSTRACT: Biodesulfurization (BDS) has emerged as an alternative to the excessively costly hydrodesulfurization of recalcitrant heterocyclic sulfur compounds, such as dibenzothiophene (DBT) and its derivatives. The model desulfurizing strain Rhodococcus qingshengii IGTS8 is responsible for the removal of sulfur through the 4S metabolic pathway, operating through a plasmid-borne dszABC operon, as well as the chromosomal gene for the flavin reductase, d szD. However, naturally occurring biocatalysts do not exhibit the required BDS activity to be useful for industrial applications and for this reason, genetic modifications are currently being explored. Here, we constructed a genetically modified R. qingshengii IGTS8 strain, which carries an additional copy of the flav

SUBMITTER: Martzoukou O 

PROVIDER: S-EPMC11783014 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature