A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1 H/³¹P magnetic resonance spectroscopy.

ABSTRACT: Awake mouse MRI and spectroscopy (MRS) are valuable techniques for studying biological questions without the confounding effects of anaesthesia. Currently, no off-the-shelf solution exists for awake mouse MRI/S. To address this, we present a Mouse Cradle Suspension System (MCSS) for awake mouse MRI/S. Our design is freely available and offers a low-cost 3D-printed setup compatible with a Bruker Biospec 94/20 scanner and commercially available 1 H/³¹P surface- and volume-coils, such as coils from Bruker Biospin (T20025V3) and Rapid (O-XL-HL-094). While the focus here is measurements in awake mouse brain, the coils and the presented setup is suitable for both mouse and rat brain, and studies of mouse body organs. Moreover, the design is easily modifiable to suit other applicati

SUBMITTER: Molhemi S 

PROVIDER: S-EPMC11783022 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature