Epitope mapping via in vitro deep mutational scanning methods and its applications.

ABSTRACT: Epitope mapping is a technique employed to define the region of an antigen that elicits an immune response, providing crucial insight into the structural architecture of the antigen as well as epitope-paratope interactions. With this breadth of knowledge, immunotherapies, diagnostics, and vaccines are being developed with a rational and data-supported design. Traditional epitope mapping methods are laborious, time-intensive, and often lack the ability to screen proteins in a high-throughput manner or provide high resolution. Deep mutational scanning (DMS), however, is revolutionizing the field as it can screen all possible single amino acid mutations and provide an efficient and high-throughput way to infer the structures of both linear and three-dimensional epitopes with high resolution.

SUBMITTER: Keen MM 

PROVIDER: S-EPMC11783119 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature