Towards the completion of Koch's postulates for the citrus huanglongbing bacterium, Candidatus Liberibacter asiaticus.

ABSTRACT: Candidatus Liberibacter asiaticus (Las) is one of the causal agents of huanglongbing (HLB), the most devastating disease of citrus worldwide. Due to the intracellular lifestyle and significant genome reduction, culturing Las in vitro has proven to be extremely challenging. In this study, we optimized growth conditions and developed a semi-selective medium based on the results of nutritional and antibiotic screening assays. Using these optimized conditions, we were able to grow Las in the LG liquid medium with ca.100- to 1000-fold increase, which peaked after 4 to 6 weeks and were estimated to contain 10⁶ to 10⁷ cells/ml. The cultured Las bacteria remained in a dynamic state of growth for over 20 months and displayed limited growth in subcultures. The sur

SUBMITTER: Zheng D 

PROVIDER: S-EPMC11783299 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature