Interrelationship between altered metabolites and the gut microbiota in people living with HIV with different immune responses to antiretroviral therapy.

ABSTRACT:

Background

Antiretroviral therapy (ART) effectively reduces opportunistic infections and mortality in people living with HIV (PLWH); however, some patients exhibit poor immune recovery. This study explores the connections among immune responses, metabolites, and the gut microbiota in PLWH with differing reactions to ART.

Methods

We analyzed the gut microbiota composition, metabolites, and immune markers in 38 PLWH who showed an immunological response (IR) and 32 who did not (INR), as classified according to CD4+ T-cell levels after 24 months of ART. Additionally, in vitro assays using cell counting kit 8, flow cytometry, and quantitative real-time reverse transcription PCR were employed to assess the effects of the metabolites on cell viability, immune marker expression, and

SUBMITTER: Tian X 

PROVIDER: S-EPMC11783423 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature