Confinement and Catalysis within <i>De Novo</i> Designed Peptide Barrels.

ABSTRACT: De novo protein design has advanced such that many peptide assemblies and protein structures can be generated predictably and quickly. The drive now is to bring functions to these structures, for example, small-molecule binding and catalysis. The formidable challenge of binding and orienting multiple small molecules to direct chemistry is particularly important for paving the way to new functionalities. To address this, here we describe the design, characterization, and application of small-molecule:peptide ternary complexes in aqueous solution. This uses α-helical barrel (αHB) peptide assemblies, which comprise 5 or more α helices arranged around central channels. These channels are solvent accessible, and their internal dimensions and chemistries can be altered predictably. Thus,

SUBMITTER: Petrenas R 

PROVIDER: S-EPMC11783595 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature