Capturing the dynamic integrity of carbocyanine fluorophore-based lipid nanoparticles using the FRET technique.

ABSTRACT: Nanoparticles capable of dynamically reporting their structural integrity in real-time are a powerful tool to guide the design of drug delivery technologies. Lipid nanoparticles (LNPs) offer multiple important advantages for drug delivery, including stability, protection of active substances, and sustained release capabilities. However, tracking their structural integrity and dynamic behaviour in complex biological environments remains challenging. Here, we report the development of a Förster resonance energy transfer (FRET)-enabled LNP platform that achieves unprecedented sensitivity and precision in monitoring nanoparticle disintegration. The FRET-based LNPs were prepared using nanoprecipitation, encapsulating high levels of 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1'-dioc

SUBMITTER: Long S 

PROVIDER: S-EPMC11783621 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature