Microscale total genomic DNA was used to explore the complete mitochondrial genomes of two biting midge species of the family Ceratopogonidae (Insecta: Diptera).

ABSTRACT:

Background

Biting midges (Ceratopogonidae, Diptera) are small insects, with body sizes ranging from 1-5 mm, and the family included 6,276 extant and 303 fossil species in 2022. But only a few mitochondrial genomes of Ceratopogonidae species have been reported, and those from several species have not been amplified. Sequencing of total or mitochondrial genomic DNA requires large amounts insect samples; however, obtaining these samples is very difficult for some insect species. The complete mitochondrial genome sequences of some insect species were cloned by long PCR, but degenerate or multiple pairs of primers were needed; thus, the long PCR method was optimized and improved in this study. The optimized method was named the two-fragment cloning method. In this study, we sequenced an

SUBMITTER: Jiang X 

PROVIDER: S-EPMC11783706 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature