Single-cell analysis identifies the interaction of altered renal tubules with basophils orchestrating kidney fibrosis.

ABSTRACT: Inflammation is an important component of fibrosis but immune processes that orchestrate kidney fibrosis are not well understood. Here we apply single-cell sequencing to a mouse model of kidney fibrosis. We identify a subset of kidney tubule cells with a profibrotic-inflammatory phenotype characterized by the expression of cytokines and chemokines associated with immune cell recruitment. Receptor-ligand interaction analysis and experimental validation indicate that CXCL1 secreted by profibrotic tubules recruits CXCR2⁺ basophils. In mice, these basophils are an important source of interleukin-6 and recruitment of the T_H17 subset of helper T cells. Genetic deletion or antibody-based depletion of basophils results in reduced renal fibrosis. Human kidney single-cell, bulk

SUBMITTER: Doke T 

PROVIDER: S-EPMC11783796 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature