<i>In vitro</i> exposure to epididymal extracellular vesicles from normospermic domestic cats improves developmental potential of sperm from teratospermic cats.

ABSTRACT: We have previously reported a difference in the composition of epididymal extracellular vesicles (EVs) between normospermic and teratospermic domestic cats. The objective of the present study was to investigate whether the fertilizing ability or developmental potential of sperm from teratospermic cats could be improved after incubation with EVs isolated from normospermic cats. For each of 11 experimental replicates, pools of EVs were collected from the whole epididymides of 5 normospermic cats (normospermic EVs). Spermatozoa were also collected from the cauda epididymides of 2 teratospermic cats, pooled, and half was co-incubated with normospermic EVs for 1 h and 15 min prior to using the sperm for in vitro fertilization (IVF). The other half of the sperm was kept for 1 h and 15 min

SUBMITTER: Sosnicki DM 

PROVIDER: S-EPMC11783848 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature