Epithelial cell diversity and immune remodeling in bladder cancer progression: insights from single-cell transcriptomics.

ABSTRACT:

Background

The progression of bladder cancer (BC) from non-muscle-invasive bladder cancer (NMIBC) to muscle-invasive bladder cancer (MIBC) significantly increases disease severity. Although the tumor microenvironment (TME) plays a pivotal role in this process, the heterogeneity of tumor cells and TME components remains underexplored.

Methods

We characterized the transcriptomes of single cells from 11 BC samples, including 4 NMIBC, 4 MIBC, and 3 adjacent normal tissues. Bulk RNA-seq data were used to validate the clinical features of characteristic cells, and protein levels of these cells were further confirmed through immunohistochemistry (IHC) and multiplex immunofluorescence.

Results

Bladder cancer progression was associated with distinct transcriptomic features in

SUBMITTER: Li J 

PROVIDER: S-EPMC11783851 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature