Usp7 contributes to the tail regeneration of planarians via Islet/Wnt1 axis.

ABSTRACT:

Background

Regeneration plays a key role in energy recycling and homeostasis maintenance. Planarians, as ideal model animals for studying regeneration, stem cell proliferation, and apoptosis, have the strong regenerative abilities. Considerable evidence suggests that ubiquitin plays an important role in maintaining homeostasis and regulating regeneration, but the function of Ubiquitin specific proteases 7 (Usp7) on regeneration in planarians remains elusive.

Methods

We identified an evolutionarily conserved gene, Usp7, and utilized RNA interference (RNAi), Quantitative real-time PCR (qRT-PCR), Whole-mount immunofluorescence, Tunnel, Whole-mount in situ hybridization (WISH), and western blotting to detect the function of Usp7 during the planarian regeneration.

Results

SUBMITTER: Liang A 

PROVIDER: S-EPMC11783867 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature