USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression.

ABSTRACT:

Background

Intracellular membraneless organelles formed by liquid-liquid phase separation (LLPS) function in diverse physiological processes and have been linked to tumor-promoting properties. The nucleolus is one of the largest membraneless organelle formed through LLPS. Deubiquitylating enzymes (DUBs) emerge as novel therapeutic targets against human cancers. However, the nucleolar phase separation of DUBs and association with lung cancer development have remained incompletely investigated till now.

Methods

GFP-USP39 fusion proteins were analyzed for LLPS properties using immunofluorescence, fluorescence recovery after photobleaching (FRAP) and in vitro LLPS assays. Intrinsically-disordered regions of USP39 were analyzed by PhaSepDB database. Transcriptomic profiling, West

SUBMITTER: Cheng S 

PROVIDER: S-EPMC11783868 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature