The effective multiplicity of infection for HCMV depends on the activity of the cellular 20S proteasome.

ABSTRACT: Human cytomegalovirus (HCMV) is a betaherpesvirus capable of infecting numerous cell types and persisting throughout an infected individual's life. Disease usually occurs in individuals with compromised or underdeveloped immune systems. Several antivirals exist but have limitations relating to toxicity and resistance. HCMV replication involves upregulation of host proteasomal activities, which play important roles in the temporal stages of replication. Here, we defined the impact on replication kinetics of the proteasome inhibitor, bortezomib. We demonstrate that bortezomib significantly reduces levels of viral genomes and infectious virions produced from a population of cells. Inhibition reduced expression of viral proteins that are influenced by genome synthesis. When added prior to 24 h

SUBMITTER: Cataldo KM 

PROVIDER: S-EPMC11784020 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature