Recombinant <i>Saccharomyces cerevisiae</i> EBY100/pYD1-FaeG: a candidate for an oral subunit vaccine against F4+ ETEC infection.

ABSTRACT: Diarrheal diseases attributable to multidrug-resistant F4+ enterotoxigenic Escherichia coli (ETEC) are escalating in severity, posing significant risks to the health and safety of both humans and animals. This study used Saccharomyces cerevisiae EBY100 to display the FaeG subunit of F4 colonizing factor as an oral vaccine against F4+ ETEC infection. Mice were orally immunized twice with 10⁸ CFU of EBY100/pYD1-FaeG, followed by a challenge with F4+ ETEC EC6 on day 7 post-immunization. The results showed that the recombinant strain EBY100/pYD1-FaeG orally enhanced the growth of the small intestine villi, significantly boosted the expression of tight junction proteins (ZO-1, Occludin, MUC2, and Claudin) (P < 0.05), and modulated the

SUBMITTER: Hu D 

PROVIDER: S-EPMC11784076 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature