Single-cell phenotyping of extracellular electron transfer via microdroplet encapsulation.

ABSTRACT: Electroactive organisms contribute to metal cycling, pollutant removal, and other redox-driven environmental processes via extracellular electron transfer (EET). Unfortunately, developing genotype-phenotype relationships for electroactive organisms is challenging because EET is necessarily removed from the cell of origin. Microdroplet emulsions, which encapsulate individual cells in aqueous droplets, have been used to study a variety of extracellular phenotypes but have not been applied to investigate EET. Here, we describe the development of a microdroplet emulsion system to sort and enrich EET-capable organisms from complex populations. We validated our system using the model electrogen Shewanella oneidensis and described the tooling of a benchtop microfluidic system for oxygen-li

SUBMITTER: Partipilo G 

PROVIDER: S-EPMC11784080 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature