Rational design and characterization of enhanced alcohol-inducible synthetic promoters in <i>Pichia pastoris</i>.

ABSTRACT: The C1 and C2 alcohols hold great promise as substrates for biomanufacturing due to their low cost and rich resources. Pichia pastoris is considered a preferred host for methanol and ethanol bioconversion due to its natural utilization of methanol and ethanol. However, the scarcity of strong and tightly regulated alcohol-inducible promoters limits its extended use. This study aimed to develop enhanced methanol- and ethanol-inducible promoters capable of improving gene expression in P. pastoris. Rational design strategies were employed to rewire the upstream regulatory sequence of the methanol-inducible P_AOX1, generating several high-strength methanol-inducible promoters with a stringent regulatory pattern. Eleven strong promoters were identified from 36 endo

SUBMITTER: Liu Q 

PROVIDER: S-EPMC11784102 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature