Identification of two new genetic loci for high-resolution genotyping of Enterocytozoon bieneusi.

ABSTRACT: In addition to the ribosomal internal transcribed spacer (ITS) locus, four loci (MS1, MS3, MS4, and MS7) have been identified to develop multilocus sequence typing tools for high-resolution genotyping of Enterocytozoon bieneusi in previous studies. However, the use of only five loci was insufficient for population genetic analysis of E. bieneusi from diverse hosts. In this study, comparison of a clinical genome sequence (C44566) with the whole genome sequence of an E. bieneusi isolate (H348) in GenBank led to the selection of the hypothetical protein 1 (hp1) and tubulin 1 (tub1) loci. Further analysis of the two loci with 156 E. bieneusi-positive samples showed high sequence polymorphisms in ITS Groups 1-6 and 10. Altogether, 30 and 23 sequence types were identified at hp1 and tub1, respec

SUBMITTER: Meng X 

PROVIDER: S-EPMC11784105 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature