About bacteriophage tail terminator and tail completion proteins: structure of the proximal extremity of siphophage T5 tail.

ABSTRACT: Bacteriophages are viruses infecting bacteria. The vast majority of them bear a tail, allowing host recognition, cell wall perforation, and DNA injection into the host cytoplasm. Using electron cryo-microscopy (cryo-EM) and single particle analysis, we determined the organization of the tail proximal extremity of siphophage T5 that possesses a long flexible tail and solved the structure of its tail terminator protein p142 (TrP₁₄₂). It allowed us to confirm the common evolutionary origin between T5 TrP_p142 and other known or putative TrPs from siphophages, myophages, and bacterial tail-like machines, despite very poor sequence conservation. By also determining the structure of the T5 tail proximal extremity after interaction with T5 bacterial receptor FhuA, we showed t

SUBMITTER: Linares R 

PROVIDER: S-EPMC11784142 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature